P53 Status Of Breast Cancer Cell Lines

Cell lines with a controversial p53 status.

P53 status of breast cancer cell lines.

I have to perform experiments with p53 wild type and mutant breast cancer cell lines for which i need to know their p53 status. Fasay detected temperature sensitive p53 mutations in breast cancer cell line bt474 and leiomyosarcoma cell line sk lms 1 and two independent p53 point mutations in sk ut 1 and sk lms 1 leiomyosarcoma cell lines. Experiments using cell culture are used for developing new diagnostic tests and new treatments for diseases. The nci 60 panel.

Breast cancer cell lines. Tp53 is the most frequently mutated gene in cancer including breast cancer. Wild type p53 was found in six cell lines and various p53 aberrations in the remaining twenty. Gastric cancer cell lines.

Bladder cancer cell lines. Brain cancer cell lines. Nsclc cancer cell lines. Can anyone tell me the p53 status wt or mutant of mcf 7 mda mb.

Germ line mutations in p53 occur in a high proportion of individuals with the li fraumeni cancer susceptibility syndrome which confers an increased risk of breast cancer this implies an important role for p53 inactivation in mammary carcinogenesis and the structure and expression of p53 has been widely studied in breast cancer. Mut sequence of the mutated codon. Tp53 codes for a transcription factor p53 that initiates transcription of genes involved in cell cycle arrest cellular senescence apoptosis metabolism dna repair and other processes following cellular stress. Cell lines with p53 mutations missense or frameshift more information is available in the handbook.

P53 activity is modulated by numerous posttranslational modifications association with protein. Leukemia lymphoma cell lines. P53 mutation in breast cancer. Using the nci 60 cell line panel as a model widely used in the literature the p53 status could not be ascertained for 13 cell lines due to a lack of homogeneous data in the literature.

Head and neck cancer cell lines. Esophageal cancer cell lines. This is a list of major breast cancer cell lines that are primarily used in breast cancer. Ever since the establishment of the first breast cancer cell line bt 20 in 1958 relatively few cell lines have been obtained due to technical difficulties in extracting viable tumor cells from the surrounding stroma 12 13 and the bottleneck of long term propagation during cultivation 12 14.

Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease. Colorectal cancer cell lines. Most cell lines were established in late 1970s. Strikingly we found discrepancies in the p53 status for 23 88 384 of cell lines for which the p53 status was established independently in two laboratories.

Codon position 1 to 393.